产品货号：
GS2162
中文名称：
Sse9I内切酶
英文名称：
Sse9I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
At 37℃ activity is 75%from maximum.To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：Sse9I内切酶，Sse9I限制性内切酶
使用说明
At 37℃ activity is 75%from maximum.To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1hourat 55℃ in a total reaction volume of 50μl. |
| Recognition sites | ↑AATT |
| 来源 | E.coli strain that carries the cloned Sse9 I gene from Sporosarcina species |
| Assayed on | pBR322 DNA |
| Optimal buffer | B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)+?BSA |
| Optimal temperature | 55℃ |
| Storage conditions | 10mMTis-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a.of enzyme for 16hoursat 55℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | MluCI,TasI,Tsp509I,TspEI |
相关搜索：Sse9I内切酶，Sse9I限制性内切酶